Optimization of Flash-Type Extraction Process for Catalpol and Stachyose from Fresh Rehmannia glutinosa
Ke Zhang
Abstract
Ke Zhang
Abstract
Objective:To optimize extraction process of catalpol and stachyose from fresh Rehmannia glutinosa.Method:With extraction rate of catalpol and stachyose as indexes,the content of catalpol and stachyose were determined by HPLC.The content of catalpol and stachyose in different extraction methods were compared,and then extraction technology was optimized by L9(34) orthogonal test.Result:Compared traditional extraction method,flash-type extraction method had higher efficiency,optimal flash-type extraction technology was as follows: extracted 3 times with 6 times the amount of 20% ethanol,each time 1 min.Conclusion:Flash-type extraction process was an efficient and rapid method for extraction catalpol and stachyose from fresh R.glutinosa.
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Objective:To optimize extraction process of catalpol and stachyose from fresh Rehmannia glutinosa.Method:With extraction rate of catalpol and stachyose as indexes,the content of catalpol and stachyose were determined by HPLC.The content of catalpol and stachyose in different extraction methods were compared,and then extraction technology was optimized by L9(34) orthogonal test.Result:Compared traditional extraction method,flash-type extraction method had higher efficiency,optimal flash-type extraction technology was as follows: extracted 3 times with 6 times the amount of 20% ethanol,each time 1 min.Conclusion:Flash-type extraction process was an efficient and rapid method for extraction catalpol and stachyose from fresh R.glutinosa.
Key concepts: Catalpol, Stachyose, Rehmannia glutinosa, Extraction (chemistry), Chemistry, Chromatography, Food science, Glycoside