Different Exercise Stages Intervention Effects on the Expression of Skeletal Muscle in Type 2 Diabetic Rats Leptin and Its Receptor Protein

Objective:1. Explore the mechanisms of glucose and lipid disorders in type 2 diabetes;2. Observed at different stages of development of type 2 diabetes exerts a long-term mechanism of aerobic exercise intervention effect and improve glucose and lipid metabolism disorders. In order to reveal the different exercise intervention in improving the effect of leptin resistance in type 2 diabetic rats and its mechanism of action to provide the experimental basis. Methods:55 weanling male rats were randomly divided into two groups,the normal control group( CON,11),high fat and high glucose group( HLG, 44). Among them,normal diet control group with normal feed,LG group given feeding high fat and high sugar diet for six weeks. More than normal weight group 25%,obesity model success,then give the intraperitoneal injection of small dose(35mg / kg) of streptozotocin(STZ),Build a model of type 2 diabetes,the successful model rats were randomly divided into two groups according to blood glucose level of the diabetic control group( DC,8),diabetic exercise group 1( DE1, 8),diabetic exercise group 2( DE2,8) and exercise diabetic group 3( DE3,8),in 1 ~ 6 weeks,7 ~ 12 weeks and 1 ~ 12 weeks swimming training,at the end of the experiment,blood glucose,insulin,leptin,skeletal muscle TG,FFA content and leptin and its receptor protein expression index. Results:Tolerance to DC group,abdominal fat weight,serum insulin,glucose,leptin and insulin were significantly higher than those in group CON;In DE1-3 group,abdominal fat weight,serum insulin,glucose and leptin were decreased in different degree,In the DE3 group decreased obviously. Muscle TG,FFA content in DC group was significantly higher than that in group CON;DE1-3 had a decreased trend compared with the DVgroup,there is significant difference only in DE3 group;And the content of TG in group DE3 were significantly lower than that of group DE2. Leptin levels in skeletal muscle of DC group increased significantly( P 0. 01),but the content of leptin receptor significantly decreased( P 0. 05);Compared with DE1-3 group and DC group,Leptin levels tended to decrease,Instead of leptin receptor content they were increased,changes in the DE3 group was statistically significant. Conclusions:1. High-fat diet with skeletal muscle in type 2 diabetic rat leptin resistance induced by lowdose STZ,To reduce the effect on the regulation of skeletal muscle lipid metabolism,lead to excessive deposition of skeletal muscle lipid,raised the overall level of leptin resistance and insulin resistance. 2. Different stages in the development of diabetes,applying the aerobic training can be improved at different extent of leptin resistance in skeletal muscle,improved glucose and lipid metabolism in the body,relieve insulin resistance and leptin resistance,in the whole process of the development of better diabetes applied exercise intervention.