Establishment of Culture System of Prunus mongolica Shoot with Single Bud

Young seedling shoots of Prunus mongolica were used as explants,using MS medium as basic medium.The explant sanitization and the effect of growth regulators in the induction,multiplication and rooting were studied.The results showed that shoots were treated by 0.1% HgCl2 for 1 min. after 70% ethanol for 10 seconds,effect was the best,rate of contamination was 18.75%,rate of survival was 81.25%.The suitable medium for the bud induction from shoot was MS+6-BA1.0 mg·L-1+NAA0.2 mg·L-1,the germination rate of bud could reach 72.2%.The optimum medium of multiplication was MS+6-BA 2.0 mg·L-1+NAA0.2 mg·L-1,rate of multiplication was 2.37.The optimum medium of rooting was 1/2MS+IBA1.0 mg·L-1,rooting rate was 40% after 14 days,mean number of roots was 3.5,mean length of roots was 2.09 cm.