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2006•China Journal of BioinformaticsRequires access

The Silico Cloning of CDC16 gene in pigs

Shi Qi-shun

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Abstract

By constructing the contigs for the express sequence tagged(EST)which were highly homologous to the human CDC16 gene(GenBank accession number:NM_-004661),the entire coding region of CDC16 gene was obtained through in silico cloning.This EST were BP436622,AJ656153,CJ022837 and BP161802.The length of the cloned cDNA was 2057 bp and 5′-untranslated region was 119 bp.The length of 3′-untranslated region was 75 bp.The length of the open reading frame(ORF)was 1863 bp.The deduced amino acid sequence was composed of 617 amino acid.It showed 97.6% and 95.3% similarity to corresponding human and mouse respectively.

About this research paper

What this paper is about

By constructing the contigs for the express sequence tagged(EST)which were highly homologous to the human CDC16 gene(GenBank accession number:NM_-004661),the entire coding region of CDC16 gene was obtained through in silico cloning.This EST were BP436622,AJ656153,CJ022837 and BP161802.The length of the cloned cDNA was 2057 bp and 5′-untranslated region was 119 bp.The length of 3′-untranslated region was 75 bp.The length of the open reading frame(ORF)was 1863 bp.The deduced amino acid sequence was composed of 617 amino acid.It showed 97.6% and 95.3% similarity to corresponding human and mouse respectively.

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Available abstract

By constructing the contigs for the express sequence tagged(EST)which were highly homologous to the human CDC16 gene(GenBank accession number:NM_-004661),the entire coding region of CDC16 gene was obtained through in silico cloning.This EST were BP436622,AJ656153,CJ022837 and BP161802.The length of the cloned cDNA was 2057 bp and 5′-untranslated region was 119 bp.The length of 3′-untranslated region was 75 bp.The length of the open reading frame(ORF)was 1863 bp.The deduced amino acid sequence was composed of 617 amino acid.It showed 97.6% and 95.3% similarity to corresponding human and mouse respectively.

Key concepts: GenBank, Open reading frame, In silico, Contig, Cloning (programming), Untranslated region, Coding region, Gene

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