Cloning Full Length cDNA of Chinese Wild Vitis Novel Gene and Sequence Analysis

According to an EST sequence of cDNA libarary from Vitis pesudoreticulata Baihe-35-1 inoculated with Uncinula necator,a pair of specific primers were designed and the SMART-RACE were performed with reverse transcription products of total RNA as the template.Concurrently bioinformatic methods were applied to analyze the obtained sequence and its induced amino acid sequence,including ORF,homologous sequences,physical and chemical properties,signal peptide,enzyme/ non-enzyme,subcellular localization and protein secondary structure.The results showed that the full length cDNA had an open reading frame of 584 bp with 107 bp 5′ untranslated regions and 162 bp 3′ untranslated regions.The deduced amino acid had 55% identity to Arabidopsis RNA/DNA binding protein,63% identity to Oryza galactosyltransferase,and the 8 to 82 amino acid was homologous with RNA recognition motif,which belongs to a typical functional domain.The protein was instable,Mr=21 801.21,pI=6.86,without signal peptide,and located to nucleus.The secondary structure was primarily composed of randon coil.