Determination of Flavonoids of Hawthorn Leaves Extract by Spectrophotometry

Objective: To study the assay method of t flavonoids in hawthorn leaves extract.Methods: Selecting rutin as control article,the contents of the flavonoids in hawthorn leaves extract that disposed through different conditions were measured and compared and analyzed by using the ultraviolet-visible spectrophotometry with two methods.Results: the concentration of the flavonoids was 86.15% by usng the Al(NO3)3(510 nm),and AlCl3(272 nm) was 35.19%.Conclusion: The concentration of flavonoids of hawthorn leaves extract had quite different by using the ultraviolet-visible spectrophotometry with two methods.