Effect of 8 - Br - cAMP on ABCA1 and MCP -1 in THP -1 cells

Objective To study the changes of ABCA1, MCP - 1 and IL - 1β after the treatment of THP1 cell lines with 8 - Br -cAMP, and to elucidate a new possible mechanism of ABCA1 gene in the formation of AS and foam cells.Method THP1 cells were resuscitated and cultured.8 - Br - cAMP(0.5mM) was added into the culture media and THP1 cells were harvested at indicated times.The mRNA and protein levels of ABCA1 , MCP - 1 and IL - 1B were investigated by real - time quantitative RT - PCR, Western blotting and ELISA.Phosphorothioate oligonucleotides of ABCA1 were used at a final concentration of 100nmol/L for all the antisense transfection experiments.The same experiments were repeated after the transfection of oligonucleotides of ABCA1.Results The mRNA and protein expressions were increased after incubation with Ox - LDL.After transfection with antisense oligonucleotides of ABCA1 , the mRNA decreased at 3 and 6 h, and the proteins decreased at 12 and 24 h.Conclusion ABCA1 increases expressions of inflammatory cytokines stimulated by 8 - Br - cAMP in THP - 1 cells and participates in atherosclerogenesis.