Significance of ABCA1 Effects on THP-1 Macrophage Inflammatory Cytokines Induced by Oxidized Low Density Lipoprotein

[Objective] To evaluate the effects of ATP binding cassette transporter A1 (ABCA1) on mRNA and protein expression of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) and protein expression of interleukin-1β (IL-1β) in THP-1 macrophage induced by oxidized low density lipoprotein (Ox-LDL) in order to investigate the mechanism that ABCA1 contributes to atherosclerogenesis.[Methods] Monocytic THP-1 cells were cultured with 100 nmol/L phorbol myristate acetate (PMA) for 72 hours to lead cells into THP-1 macrophage. Ox-LDL (30 μg/mL) was added into culture media and THP-1 macrophage cells were harvested at 3, 6, 12, and 24 hours, respectively. The mRNA and protein levels of ABCA1, ICAM-1, MCP-1 and IL-1β were investigated by real-time fluorescent quantitative RT-PCR, Western blot, and ELISA methods. After phosphorothioate oligonucleotides of ABCA1 mixture were add to culture media at final concentration of 100 nmol/L, the same experiments were repeated. [Results] The mRNA and protein amounts of ABCA1, ICAM-1, and MCP-1 and also protein amount of IL-1β were increased after macrophage incubated with Ox-LDL. Transfected with antisense oligonucleotides of ABCA1, the expressive levels of mRNA were decreased at 3 and 6 hours (P 0.01), and protein at 12 and 24 hours (P 0.01). [Conclusion] ABCA1 could increase the expression of inflammatory cytokines in macrophage induced by Ox-LDL and contributed to atherosclerogenesis.