Study on Tissue Culture of Jackfruit

An in vitro cultrue method for clonal propagation of a high quality strain of jackruit has been achieved,using shoot buds as explants.Results showed that,selection of explant collecting seasons was critical in preventing contamination and brownization,form late March to early May which was the best seasons.Addition of 2.0 mg/L 6-BA and 1.0 mg/L KT and 0.5 mg/L GA3 to MS nutrient medium induced maximum number of shoot buds(44.6%),higher KT concentration helped to induce high buds(40%).These shoots proliferated best in MS medium added BA1.5 mg/L,KT0.1 mg/L and GA3 0.5mg/L,with a rate of 2.61.High concentrations of sugar(40 g/L) helped to shoot proliferation,with an average rate of 2.43.For rooting,1/2MS+IBA1.5mg/L+NAA0.2mg/L 1.5 was the suitable medium.Rooting began 8d after implantation,with a rooting rate of 15.8%.15d later,root length was 3 to 4cm,average root number was 3-4.