Construction of Fusion Expression Vector and Expression of HBsAg/EGFP in COS-7 Cells

Objective To construct a HBsAg and EGFP fusion gene and express the gene in COS 7 cells.Methods Amplify HBsAg gene by PCR and inster the gene into plasmid pEGF P 3 1.Transfect CDS 7 cells with the recombinant plasmid pGHBs 3.1,detect the expressed HBsAg by ELISA and observe the expressed GFP under fluorescence microscope.Results HBsAg gene was detected in the transfected COS 7 cells.The expressed HBsAg was detected in the culture supernatant of the transfected cells,meanwhile the expressed GFP was observed under fluorescence microscope.Conclusion The HBsAg and EGFP fusion gene was expressed in COS 7 cells.The expressed fusion protein showed double activity of HBsAg and GFP.GFP can be used as a reported gene for tracing the expression HBsAg gene.