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1990•Acta Scicentiarum Naturalum Universitis PekinesisRequires access

Using bMT-Ia Gene as Selective Marker to Express HBsAg in a BPV Vector

Ren Gui-fang

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Abstract

Fused hepatitis B suface antigen gene with Mouse MT promoter and constructed plasmid pBMT3XR. Being used Calcium Phosphate Precipitation technique to transform C127 cells with this plasmid, HBsAg is expressed. The experiment also shows the expression of HBsAg can be induced by cadmium.

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What this paper is about

Fused hepatitis B suface antigen gene with Mouse MT promoter and constructed plasmid pBMT3XR. Being used Calcium Phosphate Precipitation technique to transform C127 cells with this plasmid, HBsAg is expressed. The experiment also shows the expression of HBsAg can be induced by cadmium.

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Available abstract

Fused hepatitis B suface antigen gene with Mouse MT promoter and constructed plasmid pBMT3XR. Being used Calcium Phosphate Precipitation technique to transform C127 cells with this plasmid, HBsAg is expressed. The experiment also shows the expression of HBsAg can be induced by cadmium.

Key concepts: HBsAg, Plasmid, Vector (molecular biology), Molecular biology, Virology, Gene, Biology, Hepatitis B virus

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