Expression of TLR-4,TNF-α and NF-κB in Rat′s Cardial Muscles during Ischemia-reperfusion

Objective To investigate the expression of TLR-4 、TNF-α and NF-κB in rat cardial muscles during ischemia reperfusion.Methods Twenty-five female SD rats were randomly divided into sham operation group(n=5),ischemia group(n=5),ischemia reperfusion 30min group(n=5),ischemia reperfusion 60 min group(n=5),ischemia reperfusion 90min group(n=5),Expression mRNA of TLR4 、TNF-α、NF-κB were measured by RT-PCR.Results The expression level of TLR4 、TNF-α and NF-κB significantly increased after ischemia-reperfusion.there are positive correlation among them.Conclusion These data suggest that myocardial ischemia-reperfusion can result in intensive activation of TLR-4 、TNF-α and NF-κB Treatment with inhibitors to TLR4 may be benefit for attenuation of myocardial injury induced by ischemia-reperfusion and subsequent acute inflammatory response.