EFFECTS OF ARSENIC TRIOXIDE ON APOPTOSIS AND CELL CYCLE OF HUMAN NASOPHARYNGEAL CARCINOMA CELL LINE

Objective To investigate the effects of arsenic trioxide (As 2O 3) on the cell cycle and apoptosis of human nasopharyngeal carcinoma cell line. Method Cell morphology, flow cytometry and TUNEL were used to examine the cell apoptosis of human nasopharyngeal carcinoma cell line CNE1 indued by arsenic trioxide.The cell cycle was determined by using flow cytometry. Result Arsenic trioxide induced CNE 1cell apoptosis with typical morphological features, and the apoptotic peak detected by flow cytometric analysis showed a dose-dependent effect. FCM data showed that the G 2/M phase ratios were increased significantly in a dose-dependent manner.Conclusion As 2O 3 could significantly induce CNE1 cell lineapoptosisand alter the cell cycle progression.