Experinental study on pulmonary injury in severe acute nectotizing pancreatitis induced by L-arginine in rats

Objective To establish the model of acute nectotizing pancreatitis (ANP) -associated lung injury induced by L-arginine in rats.Methods Forty-eight Spraque-Dawley male rats were randomly divided into tow groups C and A.ANP and APALI were induced by injected intraperitoneaUy three times with 1.5 mg/g body weight 6% L-Arg in an interval of 1 hour in group A.Group C received saline alone on the same methods.Rats were killed at the 6th, 12th and 24th hour after the last time of L-Arg injection.The pathological changes of tissue of pancreatic and lung were respectively analyzed and scored according to Kusser's and Lei weizhang's standard.Results With time going,the pathological injuries of the pancreas and lung in group A were significantly more severely than that in group C(P0.01).The serum amylase in group A was significantly higher than that in group C, especially at the 24th hour(P 0.01).Pathological injury score in pancreatic showed a correlation with the level of amylase and the lung injury ( r, = 0.374, P = 0.046; r, = 0.452, P = 0.027 ).Conclusion The rat model of acute nectotizing pancreatitis (ANP) -associated lung injury can be induced by intraperitoneal injection of L-arginine(l.5 mg/g,three times) at the concentration of 60g/L at an interval of 1 hour.Lung injury is closely related to pancreatic tissue injury in ANP.