Optimization of Ultrasonic Extraction Parameters and Deprotein Process for Polysaccharide from Sijunzi Decoction

Objective:To optimize the parameters of ultrasonic extraction and deprotein process for polysaccharide of sijunzi decoction. Method:With the extraction temperature,extraction time,extraction times as three factors,orthogonal experiment of three levels were designed to optimize the parameters of ultrasonic extraction for polysaccharide of sijunzi decoction. Sevag method and Sevag-enzyme method were compared for deprotein of polysaccharide of sijunzi decoction,and single-factor experiments were designed. Result:The optimal conditions were that polysaccharide of sijunzi decoction was extracted at 80°C,30 min for 3 times. Sevag-enzyme method was better for deprotein than Sevag method, and the optimum condition was that the amount of trypsin was 1%,with water bath at 37 ℃,enzymolysis for 3 hr,and processed with Sevag agent for 3 times.Conclusion:The parameters of ultrasonic extraction and deprotein process for polysaccharide of sijunzi decoction were optimized,and it could be the basis for the further isolation and activity study.