Optimization of Extraction Technology for Modified Sijunzi Decoction by Orthogonal Design

Objective: To optimize extraction technology of modified Sijunzi decoction. Method: Taking total extraction amounts of ginsenoside as index, L 9( 34) orthogonal design was adopted to optimize alcohol extraction technology of Ginseng Radix et Rhizoma,Atractylodis Macrocephalae Rhizoma and Paeoniae Radix Alba with ethanol amount and concentration,extraction time and times as factors; With yield of total polysaccharides as index,L 9( 34) orthogonal design was used to optimize water extraction technology of Poriae Cutis,Hedyotidis Herba and Glycyrrhizae Radix et Rhizoma by taking solid-liquid ratio,extraction time and times as factors. The content of ginsenoside was determined by HPLC, mobile phase of acetonitrile-water( 13 ∶ 87), detection wavelength 203 nm; UV was adopted to measure the content of polysaccharides with detection wavelength at 489 nm. Result: Optimum alcohol extraction process was as following: extracted twice with 6 times the amount of 70% ethanol for 1. 5 h per time; Extraction amount of ginsenoside was up to 7. 69 mg·g- 1. Optimum water extraction process was: extracted three times with 8 times the amount of water for 2 h each time; Yield of total polysaccharides reached 2. 18%. Conclusion: This optimized extraction technology was stable and feasible,it could full extract alcohol-soluble and water-soluble active ingredients from modified Sijunzi decoction.