Effect of DIT on cytokines secretion in cultured human thyrocytes

Objective To investigate the influence of DIT on cytokines(IL-6,IL-8) secretion of human thyrocytes and compare DIT with KI.To study a good kind of supplement to USI that is safe and effective.Methods The human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10-8～10-3 mol/L DIT or KI.The contents of cytokines such as IL-6,IL-8 in supernatant of cultured thyrocytes were detected by ELISA method.Results A few IL-6,IL-8 were detected in supernatant of normal thyrocytes.IL-6 in supernatant of DIT group(10-6～10-3 mol/L) increased significantly compared with normal thyrocytes.L-6 in supernatant of DIT group(10-4 mol/L,10-3 mol/L)decreased significantly compared with KI group(10-4 mol/L,10-3 mol/L).IL-8 in supernatant of DIT group(10-5～10-3 mol/L) increased significantly compared with normal thyrocytes.IL-8 in supernatant of DIT group(10-5 mol/L,10-4 mol/L,10-3 mol/L) decreased significantly compared with KI group(10-5 mol/L,10-4 mol/L,10-3 mol/L).In the same contents,IL-6,IL-8 in supernatant of DIT group decreased significantly compared with KI group.Conclusion High concentration of iodine can increase cytokines(IL-6,IL-8) secretion of normal thyrocytes.And DIT has more security than KI.