Influence of organic iodine and inorganic iodine on thyrocytes apoptosis

Objective To research the influence of organic iodine(DIT) and inorganic iodine(KI) on cellular morphology and the expression of a gene family about apoptosis(Bcl-2 and Bax).Methods The human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were cultured.They were divided into control group(medium),KI group(the dosages of KI were 10-7,10-5 and 10-3 mol/L respectively) and DIT group(the dosages of DIT were 10-7,10-5 and 10-3 mol/L respectively).Various dosages of DIT and KI diluted with medium were applied in the culture for 48 h.The local distribution and expression of Bcl-2 and Bax of human thyroid cell were detected by immumohistochemistry after different dosages of DIT and KI were applied.Results The expression of Bcl-2 significantly decreased in KI group(l0-5,10-3 mol/L) and DIT group(10-3 mol/L),and the expression of Bax significantly increased in KI group(l0-5,10-3 mol/L) and DIT group(l0-5,10-3 mol/L) than that in the control group(P0.05,P0.01);As the level of exposure increased,the expression of Bcl-2 decreased and the expression of Bax increased in degrees.At the same level of DIT and KI,the expression of Bcl-2 of DIT was significantly higher and the expression of Bax of DIT was significantly lower(P0.05,P0.01).Conclusion High level iodine exposure may cause morphological changes and apoptosis of thyrocytes and the effect of DIT may be less compared with KI.