Studies on the affinity membrane for adsorption and purification of lysozyme (III) New method of purificating lysozyme from enzyme mixture and egg white (shell)

The new technique using metal ions chelated affinity membrane to purificate lysozyme from enzyme mixture and egg white (shell) was studied. The results showed that the affinity membrane had good selectivity adsorption performance to lysozyme, but less adsorption to glucose oxidase. Also the affinity was used to extract lysozyme from egg white (shell) directly. The orthogonal test was designed to find the optimal operating conditions. Under this optimal conditions the purity of lysozyme was larger than 17?500?U/mg.