Mechanism of Blocking Cervical Precancerous Lesions with Astragalus Membranaceus

Objective To study the mechanism of different concentration of astragalus membranaceus injection on inhibiting the proliferation and apoptosis of cervical immortalized cells( H8) in vitro. Methods MTT method was used to detect the effect of inhibiting proliferation of different concentrations of astragalus membranaceus injection( 100,200,500 and 1 000 g / L) in vitro on H8 cells. AnnexinV-FITC / PI method was used to detect the apoptosis rate of H8 cells 48 hours after treated with different concentrations of astragalus membranaceus injction. Western blotting method was applied to detect the expression of apoptosis related protein Bcl-2,Bax and p53. Results The results of MTT method showed that different concentrations of astragalus membranaceus injection had significant inhibitory effect on H8 cells in vitro and the effect was dependent on time and concentration. The inhibition rate of each group showed statistically significant differences( Finter-group= 4. 782,Ftime= 5. 539,P 0. 05). The results of Annexin V-FITC / PI method showed that different concentrations of astragalus membranaceus injection could all cause apoptosis. 48 h after the intervention of astragalus membranaceus injection,the apoptosis rate was( 4. 492 ± 1. 084) % in the control group,( 18. 054 ± 1. 108) % in the 100 g / L group,( 29. 368 ± 1. 115) % in the 200 g / L group,( 37. 090 ± 2. 652) % in the 500 g / L group and( 54. 614 ± 3. 052) % in the 1 000 g / L group. The difference between each group was statistically significant( F = 3. 782,P 0. 05). The results of Western blotting method showed that the increase of concentration could cause the decrease of the expression of Bcl-2 protein, and the increase of the expression of Bax and p53 protein. Conclusion The astragalus membranaceus injection can obviously inhibit and induce the apoptosis of H8 cells,and the mechanism may relate to the decreased expression of Bcl-2 protein and increased expression of Box and p53 protein.