The growth inhibitory effect of Astragalus Injection to the colon cancer cell line SW480

Objective: To study the growth inhibitory effect and mechanism of Astragalus Injection to apoptosis of colon cancer SW480 cell lines.Methods: The control group,the oxaliplatin control group and the experimental group were included in this study.The influence of Astragalus Injection on the growth proliferation and apoptosis of SW480 cell in vitro was detected with MTT assay.The apoptosis was detected with flow cytometry(FCM) and PI staining.Results: The inhibition of the proliferation of SW480 cell lines in experimental group was more obviously compared with the control group,and which was directly proportional to the action concentration(P=0.0000.01).FCM showed that the apoptosis rates in groups of 125 μg/ml,250 μg/ml,500 μg/ml,1 000 μg/ml were 24.2%,42.6%,64.1%,84.0% respectively after Astragalus Injection on SW480 cell after 48 hours.And which were significantly higher than the control group,the differences were significant(P=0.0000.01).Conclusion: Astragalus Injection can inhibit the growth of SW480 cell and induce apoptosis of colon cancer cell SW480.