Cloning and sequencing of human brain thioredoxin reductase

Objective:To clone the thioredoxin reductase (TR) gene from human fatal brain. Methods: The total RNA was extracted from human fatal brain; TR cDNA was obtained by RT-PCR method,and was cloned into pGEM-T vector, and then was transformed into E. Coli (DH5a); The positive transformant was identified with 2 groups of restriction en-donucleases; The positive clones was purified and sequenced. Results: The DNA sequence has been determined. Of the 1 513 base pairs sequenced, 1 500 base pairs were in an open reading frame encoding a mature protein with 500 amino acids; Identity to DNA sequence of TR gene from human placenta was 99% . There were 5 differences in the base pairs, and 4 differences in the amino acid sequences. Conclusion:The Chinese brain TR gene has been obtained firstly by us.