Molecular cloning and sequence analsis of the thioredxin reductase from humanleuxocyte
Sun Lu
Abstract
Sun Lu
Abstract
To clone and sequence analysis thioredxin redutase cDNA from human leucocyte.Methods:TR cDNA was obtained with RT-PCR and positive cloning was purified and sequenced. Results: of the 1 554 bp, I 494 bp were in an open reading frame encoding mature protein with 498 amino acids. Conclusion:The DNA encoding human leucocyte thioredxin reductase has been determined and the amino acid sequence has been predicted.
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To clone and sequence analysis thioredxin redutase cDNA from human leucocyte.Methods:TR cDNA was obtained with RT-PCR and positive cloning was purified and sequenced. Results: of the 1 554 bp, I 494 bp were in an open reading frame encoding mature protein with 498 amino acids. Conclusion:The DNA encoding human leucocyte thioredxin reductase has been determined and the amino acid sequence has been predicted.
Key concepts: Complementary DNA, Open reading frame, Cloning (programming), clone (Java method), Biology, Peptide sequence, Amino acid, Molecular biology