Influences of pioglitazone on the appearance of protein tyrosine phosphatase 1B in the pancreas of high fat diet induced insulin resistance rats

Objective To observe the effect of Pioglitazone on the appearance of protein tyrosine phosphatase 1B in the pancreas of insulin resistance rats.Methods 60 SD rats were randomly divided into the control group(NC group,n=10) on regular diet,the high fat diet group(HFN group,n=15) and the high fat diet group administrated with Pioglitazone(HFP group,n=15).The last two groups(HF groups) were on high fat diet.After twelve weeks,verify the features of the models.The HFP group were treated with Pioglitazone [30 mg/(kg·d),intragastric administration] daily for 2 weeks.The NC group and the HFN group received a saline intragastric administration of equal volume.The expression of PTP-1B in the pancreas of rats was detected by immunohistochemistry and Western blot.Results The sensitivity of insulin was degraded in the HF group than that in the NC group(P0.01).The immunohistochemistry and Western blot test showed that the expression of PTP-1B protein in the HF group was higher than that in the NC group(P0.01).The expression of PTP-1B protein in the HFP group significantly decreased compared with that in the HFN group(P0.01).Conclusion The obese rats induced by high fat diet show insulin resistance.At the same time,the PTP-1B level in their pancreas increase.Pioglitazone could degrade the expression of PTP-1B in the pancreas and improve the insulin resistance.