Antioxidant Activity of Polysaccharide from the Leaves of Acanthopanax Sessiliflorus Seem.
Zheng You-lan
Abstract
Zheng You-lan
Abstract
Study on the antioxidant activity from different parts of Acanthopanax sessiliflorus(Rupr.et Maxim) Seem.It is necessary that we explore it as dietary antioxidants and suggest feasibility suggestion.Using ABTS ·+,DPPH · radical and reducing reaction,determined the antioxidant capacity and the phenolics content of the total extracts and different parts of the plant in different concentrations by spectrophotometric method.The different parts of leaves had the radical scavenging on DPPH ·,ABTS ·+ and reducing power on Fe3+ significantly.The result showed a good dose-concentration relationship.30% part has the best antioxidant activities at the concentration of 0.62 mg/mL.This part showed 99.588%.In the DPPH · radical scavenging;and 99.387% in the ABTS ·+ radical scavenging,while the radical scavenging closed to the same concentration of Vc.The IC50 were 0.024 mg/mL,0.127 mg/mL,respectively.The phenolics content of 30% part was highest which was 0.567%.The leaves have a significant antioxidant activity which has a relationship with phenolics content.The result of this paper objectively showed that it was precise to determine the antioxidant activity of polysaccharide from the leaves of Acanthopanax sessiliflorus by DPPH,ABTS and reducing power.
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Study on the antioxidant activity from different parts of Acanthopanax sessiliflorus(Rupr.et Maxim) Seem.It is necessary that we explore it as dietary antioxidants and suggest feasibility suggestion.Using ABTS ·+,DPPH · radical and reducing reaction,determined the antioxidant capacity and the phenolics content of the total extracts and different parts of the plant in different concentrations by spectrophotometric method.The different parts of leaves had the radical scavenging on DPPH ·,ABTS ·+ and reducing power on Fe3+ significantly.The result showed a good dose-concentration relationship.30% part has the best antioxidant activities at the concentration of 0.62 mg/mL.This part showed 99.588%.In the DPPH · radical scavenging;and 99.387% in the ABTS ·+ radical scavenging,while the radical scavenging closed to the same concentration of Vc.The IC50 were 0.024 mg/mL,0.127 mg/mL,respectively.The phenolics content of 30% part was highest which was 0.567%.The leaves have a significant antioxidant activity which has a relationship with phenolics content.The result of this paper objectively showed that it was precise to determine the antioxidant activity of polysaccharide from the leaves of Acanthopanax sessiliflorus by DPPH,ABTS and reducing power.
Key concepts: DPPH, ABTS, Chemistry, Antioxidant, Scavenging, Food science, Polysaccharide, Traditional medicine