Study on Antioxidative Properties of Different Fractions from Acanthopanax Senticosus(Rupr.etMaxim.) Harms Leaves

Objective Study on different Fractions of Acanthopanax Senticosus(Rupr.etMaxim.) Harms Leaves at different concentrations,and determine its antioxidative activities.Methods The antioxidative activities of Acanthopanax Senticosus compared with VC were investigated employing various methods established in vitro systems such as DPPH(1,1-diphenyl-2-picrylhydrazyl) radical ABTS(2,2-azo-bis-(3-ethylbenzothiozoline-6-sulphonic acid) radical scavenging assay and reducing power assay measured by ultraviolet spectro-photometer.Results The elution fractions of Acanthopanax Senticosus exhibited significant antioxidant properties on ABTS free radical,DPPH radical scavenging systems,and good reduction.The inhibition of freeing radical scavenging ability of the different elution fractions from strong to weak is: 30%fraction10%fraction the total extracts50%fraction.The antioxidant properties of different fractions depended well on the their dose.With the increasing of the concentration various fractions,the antioxidative capacity was enhanced.The inhibition of ABTS radical scavenging was 99.541% at the concentration of 0.64mg/mL in 30% fraction,and the inhibition of DPPH radical scavenging was 95.331% at the same concentration in 30% fraction,both close to VC.The inhibition in 10% fraction was weaker than 30% fraction,it was 96.324%、88.033% respectively.In the reducing power assay,the absorbance was about closed to VC that was 2.269 of 30% fraction,and the 10% fraction was 2.155.Conclusion Different fractions of Acanthopanax Senticosus showed significant antioxidant properties,the 30% ethanol fraction exhibited highest antioxidant activities in different investigation systems.The present studies offered a scientific record that 30% ethanol fraction would be developped to be an antioxidant.