ScholarLens Back to search
2010•Food and MachineryRequires access

Preparation of artificial antigen and polyclonal antibodies against deoxynivalenol

Shuo Wang

Open publisher page 1 citations

Abstract

Deoxnivalenol hapten was synthesized successfully followed by studying of the two kinds of hapten synthesis method. The immunogen was prepared following a scheme that employs active ester method for the conjugation of the haptens to BSA(bovine serum albumin), with which polyclonal antibodies against deoxynivalenol were raised in rabbits after immunization. The antiserum titer was 1∶32 000, and the antiserum was purified by proteinA-sepharose 4B immunity affinity chromatography. The antibody specificity was checked on the basis of the 50% inhibition levels, relative cross-reactivities with DON, 3-AC-DON, 15-AC-DON, T-2, OTA and ZEN were found to be 100%, 500%, 2.50%, 0.10%, 0.01%, 0.01%.

About this research paper

What this paper is about

Deoxnivalenol hapten was synthesized successfully followed by studying of the two kinds of hapten synthesis method. The immunogen was prepared following a scheme that employs active ester method for the conjugation of the haptens to BSA(bovine serum albumin), with which polyclonal antibodies against deoxynivalenol were raised in rabbits after immunization. The antiserum titer was 1∶32 000, and the antiserum was purified by proteinA-sepharose 4B immunity affinity chromatography. The antibody specificity was checked on the basis of the 50% inhibition levels, relative cross-reactivities with DON, 3-AC-DON, 15-AC-DON, T-2, OTA and ZEN were found to be 100%, 500%, 2.50%, 0.10%, 0.01%, 0.01%.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Deoxnivalenol hapten was synthesized successfully followed by studying of the two kinds of hapten synthesis method. The immunogen was prepared following a scheme that employs active ester method for the conjugation of the haptens to BSA(bovine serum albumin), with which polyclonal antibodies against deoxynivalenol were raised in rabbits after immunization. The antiserum titer was 1∶32 000, and the antiserum was purified by proteinA-sepharose 4B immunity affinity chromatography. The antibody specificity was checked on the basis of the 50% inhibition levels, relative cross-reactivities with DON, 3-AC-DON, 15-AC-DON, T-2, OTA and ZEN were found to be 100%, 500%, 2.50%, 0.10%, 0.01%, 0.01%.

Key concepts: Immunogen, Hapten, Polyclonal antibodies, Antiserum, Bovine serum albumin, Affinity chromatography, Chemistry, Antibody

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Preparation of artificial antigen and polyclonal antibodies against deoxynivalenol — Research Paper | ScholarLens