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Production and characterization of monoclonal antibody against deoxynivalenol

Zhang Zhaowei

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Abstract

Deoxynivalenol(DON) was activated by 1,1-dicyclohexylcarbodiimide(CDI),and conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) as immunogen(DON-BSA) and coating antigen(DON-OVA).After fusing mouse myeloma cells(SP 2/0) with spleen cells from Balb/c mice which were immunized by the artificial antigen conjugated with BSA,screening and subclonal procedure,one hybridoma cell line 1D5 stably secreting monoclonal antibody(McAb) against DON was obtained.Isotype of the monoclonal antibody 1D5 was shown as IgG1.The results showed that the affinity constant was 8.33×107 L/mol,and no cross-reactivity was seen to the other tested mycotoxins(Fumonisin B1,Ochratoxin A,T-2 toxin,Zearalenone).The antibody also showed excellent stability,which could provide a key reagent material for development deoxynivalenol-specific immuno-assay technologies and products.

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What this paper is about

Deoxynivalenol(DON) was activated by 1,1-dicyclohexylcarbodiimide(CDI),and conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) as immunogen(DON-BSA) and coating antigen(DON-OVA).After fusing mouse myeloma cells(SP 2/0) with spleen cells from Balb/c mice which were immunized by the artificial antigen conjugated with BSA,screening and subclonal procedure,one hybridoma cell line 1D5 stably secreting monoclonal antibody(McAb) against DON was obtained.Isotype of the monoclonal antibody 1D5 was shown as IgG1.The results showed that the affinity constant was 8.33×107 L/mol,and no cross-reactivity was seen to the other tested mycotoxins(Fumonisin B1,Ochratoxin A,T-2 toxin,Zearalenone).The antibody also showed excellent stability,which could provide a key reagent material for development deoxynivalenol-specific immuno-assay technologies and products.

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Available abstract

Deoxynivalenol(DON) was activated by 1,1-dicyclohexylcarbodiimide(CDI),and conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) as immunogen(DON-BSA) and coating antigen(DON-OVA).After fusing mouse myeloma cells(SP 2/0) with spleen cells from Balb/c mice which were immunized by the artificial antigen conjugated with BSA,screening and subclonal procedure,one hybridoma cell line 1D5 stably secreting monoclonal antibody(McAb) against DON was obtained.Isotype of the monoclonal antibody 1D5 was shown as IgG1.The results showed that the affinity constant was 8.33×107 L/mol,and no cross-reactivity was seen to the other tested mycotoxins(Fumonisin B1,Ochratoxin A,T-2 toxin,Zearalenone).The antibody also showed excellent stability,which could provide a key reagent material for development deoxynivalenol-specific immuno-assay technologies and products.

Key concepts: Immunogen, Chemistry, Monoclonal antibody, Bovine serum albumin, Ochratoxin A, Zearalenone, Antigen, Antibody

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