Screening of A High-efficiency Cellulose-decomposing Bacteria Strain and Study on Its Cellulase-producing Conditions

5 high-efficiency cellulose-decomposing bacteria strains were isolated from liquor spent grains,among them,Trichoderma viride strain S7 with the strongest decomposing capability was used as the starting strain for single factor optimization test of its cellulase-production conditions by solid fermentation,and the optimized conditions were summed up as follows: the optimum mass ratio of spent grains powder and wheat bran was 4∶1 in solid culture mediums,ammonium sulphate content was 2 %,initial pH value was 4.0,and 96 h fermentation at 30～35 ℃,finally,the activities of carboxymethyl cellulase and filter paper enzyme could reach up to 57.08 μg/minomL and 26.02 μg/minomL respectively.