Study on the Expression of mRNA for NSE、GFAP and S 100 Protein in the Brain of Newborn Rat with Hypoxic ischemic Damage in Newborn Rat

Objective To observe the expression of mRNA for neuron specific enolase (NSE), glial fibrillary acidic protein(GFAP)and S 100 protein (S 100) in cerebral tissue after hypoxic ischemic brain damage(HIBD) in newborn rat. Methods The dynamic changes of expression of mRNA for NSE、GFAP and S 100 protein in the cerebral tissue were studied by using 7d old SD rat hypoxic ischemic brain damage model and RT PCR technique. Results The peak expression of mRNA for NES and for GFAP and S 100 protein occurred 24 and 48 hours after HIBD respectively, and their expression decreased gradually. Conclusion The expression of mRNA for NSE、GFAP and S 100 protein increased significantly after HIBD. It suggests that the increased level of NSE、GFAP and S 100 protein in serum and cerebrospinal fluid (C.S.F) maybe reflect the increase of transcription and transnational except for the leakage of damaged cell.