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Separation of Total Flavonoids from Honeysuckle Flower by Two-phase Aqueous System Synergism with Ultrasound Extraction

Wu Dao

Abstract

Total flavonoids from honeysuckle flower was separated by alcohol-ammonium sulfate two-phase aqueous system synergism with ultrasound extraction.Influence of extraction time,material-solvent ratio,ethanol-water ratio and salt concentration on extraction ratio of total flavonoids was researched by single factor experiment and orthogonal test.The results showed that using ethanol-water ratio for 0.7(V/V),ammonium sulfate concentration for 0.27g/mL preparation two-phase aqueous solution,extraction yield of total flavonoids is 1.97% in material-solvent ratio for 1:25(m/V),60℃ the ultrasonic extraction 75min.

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Key concepts: Honeysuckle, Chemistry, Extraction (chemistry), Chromatography, Ammonium sulfate, Aqueous solution, Yield (engineering), Solvent