Callus Induction and Establishment of Cell Suspension Culture System for Peanut

With Zhonghua2as material,the optimum culture conditions of callus induction and cell suspension culture were studied,the growth characteristics of suspension cell were detected,and a cell suspension culture system was established.The results showed that leaf was the best explant for callus induction and cell suspension culture,the optimal medium for callus induction was MS+6-BA 2.0mg / L+2,4-D 2.0mg / L,the optimal formula for suspension culture was MS+6-BA 2.0mg / L+ 2,4-D 2.0 mg / L+KT 1.5 mg / L.The growth of suspension cells exhibited an S-shaped curve,growth mass reached the maximum after 12dculture,the optimal subculture cycle was around 12d.In the process of suspension culture,the suspension cell number exhibited the change trend : firstly increased,then decreased.The change trends of pH value and conductivity showed the same : firstly decreased,then increased,and then became stable.