Production of Biodiesel Fuel from Cottonseed Oil Catalyzed by Immobilized Lipase

Contrasting the lipase absorbability of different carriers,D101 was screened as the carrier.Absorbed on D101,the lipase was then cross-linked by glutaraldehyde.Compared with the free lipase,the stability of immobilized lipase was improved in certain degree.The optimal pH and temperature of immobilized one was 8 and 50℃,respectively.Immobilized lipase was used to catalyze the transesterification of cottonseed oils for the production of biodiesel.The optimal reaction condition was obtained in the octane,where the mass ratio of the lipase and carriers dosage was 0.48,the molar ratio of methanol and cottonseed oil was 3:1,and the initial water content was about 1.25%(V/V),respectively.Under the pH of 8 and the temperature of 40℃,the conversion of biodiesel reached 65% in 24h.