2009Chemistry & BioengineeringRequires access

Study on Preparation of Peanut Peptides from Peanut Meal Catalyzed by Two Enzymes

XU Hai-li

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Abstract

The functionality small peptide was prepared by hydrolysis of peanut meal catalyzed by two enzymes of AS1.398 neutral protease and papain,and the hydrolysis process parameters were optimized.The sequence of the factors on degree of hydrolysis of protein was reaction temperaturepH valuesubstrate concentrationenzyme dosagereaction time.The optimum enzymolysis conditions were as follows:the substrate concentration was 3.5%,the reaction temperature was 45℃,the enzyme dosage was 12 500 U·g-1,the pH value was 7.5,the reaction time was 3.0 h.

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The functionality small peptide was prepared by hydrolysis of peanut meal catalyzed by two enzymes of AS1.398 neutral protease and papain,and the hydrolysis process parameters were optimized.The sequence of the factors on degree of hydrolysis of protein was reaction temperaturepH valuesubstrate concentrationenzyme dosagereaction time.The optimum enzymolysis conditions were as follows:the substrate concentration was 3.5%,the reaction temperature was 45℃,the enzyme dosage was 12 500 U·g-1,the pH value was 7.5,the reaction time was 3.0 h.

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Available abstract

The functionality small peptide was prepared by hydrolysis of peanut meal catalyzed by two enzymes of AS1.398 neutral protease and papain,and the hydrolysis process parameters were optimized.The sequence of the factors on degree of hydrolysis of protein was reaction temperaturepH valuesubstrate concentrationenzyme dosagereaction time.The optimum enzymolysis conditions were as follows:the substrate concentration was 3.5%,the reaction temperature was 45℃,the enzyme dosage was 12 500 U·g-1,the pH value was 7.5,the reaction time was 3.0 h.

Key concepts: Chemistry, Papain, Hydrolysis, Enzyme, Catalysis, Substrate (aquarium), Protease, Enzymatic hydrolysis

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