2000•PubMedRequires access

[Construction and expression of recombinant adeno-associated HIV -1 virus].

Yongjun Guan, Liu H, Y Zhu

Abstract

OBJECTIVE: Plasmid of AAV vector with HIV-1 gag and gp 120 were constructed respectively. METHODS: The recombinant AAV-HIV gag and gp120 viruses were obtained by co transfection of AAV-HIV plasmid and Adenovirus 5 in 293 cells. RESULTS: The recombinant AAV-HIV virus expressed well and the titer of the recombinant viruses were between 10(4)-10(5). CONCLUSIONS: The recombinant AAV-HIV virus can be used to infect the target cells for detection of cellular immunity and for vaccine studies.

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Key concepts: Recombinant DNA, Virology, Adeno-associated virus, Plasmid, Virus, Recombinant virus, Titer, Biology