2012•Journal of Southwest China Normal UniversityRequires access

Tracing Insecticidal Ingredients from Buddleja albiflora Hemsl.

Xing Zhang

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Abstract

The petroleum extracts and ethyl acetate extracts of 95% ethanol extract of Buddleja albiflora H.were isolated by means of phytochemistry combined with insecticidal bioassay.Fraction AG from petroleum extracts,with concentration of 5 mg/mL,showed the antifeedant rate against 3rd instar of Mythimna separata W.of 58.44%,79.77% and 71.52% during 24,48 and 72 h,respectively,which were 89.03% and 72.73% against 5rd instar of Pieris rapae L.during 24 and 48 h.Fraction BB from ethyl acetate extracts showed the antifeedant rate against 3rd instar of Mythimna separata W.of 97.34%,100% and 100% during 24,48 and 72 h,respectively,which were 97.33% and 99.46% against 3rd instar of Plutella xylostella L.during 24 and 48 h.The results showed that the insecticidal ingredients of petroleum extracts were distributed in AG part,while that of ethyl acetate extracts were in BA,BB and BC parts.

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The petroleum extracts and ethyl acetate extracts of 95% ethanol extract of Buddleja albiflora H.were isolated by means of phytochemistry combined with insecticidal bioassay.Fraction AG from petroleum extracts,with concentration of 5 mg/mL,showed the antifeedant rate against 3rd instar of Mythimna separata W.of 58.44%,79.77% and 71.52% during 24,48 and 72 h,respectively,which were 89.03% and 72.73% against 5rd instar of Pieris rapae L.during 24 and 48 h.Fraction BB from ethyl acetate extracts showed the antifeedant rate against 3rd instar of Mythimna separata W.of 97.34%,100% and 100% during 24,48 and 72 h,respectively,which were 97.33% and 99.46% against 3rd instar of Plutella xylostella L.during 24 and 48 h.The results showed that the insecticidal ingredients of petroleum extracts were distributed in AG part,while that of ethyl acetate extracts were in BA,BB and BC parts.

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Available abstract

The petroleum extracts and ethyl acetate extracts of 95% ethanol extract of Buddleja albiflora H.were isolated by means of phytochemistry combined with insecticidal bioassay.Fraction AG from petroleum extracts,with concentration of 5 mg/mL,showed the antifeedant rate against 3rd instar of Mythimna separata W.of 58.44%,79.77% and 71.52% during 24,48 and 72 h,respectively,which were 89.03% and 72.73% against 5rd instar of Pieris rapae L.during 24 and 48 h.Fraction BB from ethyl acetate extracts showed the antifeedant rate against 3rd instar of Mythimna separata W.of 97.34%,100% and 100% during 24,48 and 72 h,respectively,which were 97.33% and 99.46% against 3rd instar of Plutella xylostella L.during 24 and 48 h.The results showed that the insecticidal ingredients of petroleum extracts were distributed in AG part,while that of ethyl acetate extracts were in BA,BB and BC parts.

Key concepts: Mythimna separata, Instar, Plutella, Ethyl acetate, Pieris rapae, Phytochemistry, Bioassay, Chemistry

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