Intervention effect of probucol on islet function in type 2 diabetes rats

Objective To investigate the effect of antioxidant probucol on oxidative stress and secretory function of islet in type 2 diabetes rats. Methods Twenty-four rats were randomly divided into three groups:normal control group(group A) ,diabetic group(group B) and probucol-treated diabetic group(group C) ,8 in each. Rats in group C were treated with probucol for one month [500 mg/(kg/d) ,twice per day]. High-fat and high-sugar diets with a low dose injection of streptozotocin were performed in rats to establish type 2 diabetic models. The methods of glucose oxidase,enzymatic end-point and radioimmunoassay were repeatedly used to test the levels of fasting blood glucose(FBG) ,postprandial blood glucose(PBG) ,triglyceride(TG) ,total cholesterol(T-ch) and fasting insulin(FINS) . Pancreatic tissues were homogenized to test the content of malondialdehyde(MDA) ,nitric oxide(NO) ,superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) . Immunohistochemical method was used to detect the insulin expression. Results Compared with rats in the group A,the levels of MDA,NO and FINS were higher,while the levels of SOD,GSH-Px and insulin expression were lower in the group B(all P0.05) . After treated with probucol,compared with the group B,the levels of MDA,NO were lower,while the levels of SOD,GSH-Px and insulin expression were higher in the group C(all P0.05) . Conclusion Probucol can preserve pancreatic islet against damage from oxidative stress and improve the endocrine function of islet cells in type 2 diabetic rats.