Influence of Mori Folium Total Flavonoid on β-cells in Rats with Type-2 Diabetic Mellitus

Objective: To explore the effect of Mori Folium total flavonoid(MFTF) on β-cells in rats with type-2 diabetic mellitus(T2DM).Method: The rat T2DM model was replicated by streptozotocin(STZ,30 mg.kg-1 body weight) injection,the modeling rats were randomly divided into five group,the model group,the MFTF(50,100,150 mg.kg-1) groups and rosiglitazone(1.8 mg.kg-1) group.10 normal rats were taken control group.The treatment groups were orally administered once per day,model group was orally administered with distilled water.After treatment for 5 week,fasting blood glucose(FBG) and insulin(FINS) level in serum were estimated.Malondialdehyde(MDA) content,superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activity in pancreatic tissue were measured.The expression of Bcl-2/Bax in islet tissue was examined by using immunohistochemistry.The pancreatic islet β cell apoptosis was detected by TUNEL.Result: MFTF could decrease MDA level significantly and increase SOD,GSH-Px level in pancreatic tissue(P 0.05-P 0.01),increase the levels of Bcl-2 expression and decrease the expression of Bax protein significantly(P 0.05-P 0.01).Decrease pancreatic β-cell apoptosis(P 0.05-P 0.01),decrease the level of glucose,insulin and insulin resistance index(P 0.05-P 0.01),especiallythe high dose group(150 mg.kg-1) showed a best action.Conclusion: MFTF can improve insulin resistance and resists oxidative stress reaction and decrease apoptosis of pancreatic islet β cell.