Scavenging Effects of Carambola Extracts on Oxygen Radical in Vitro
Zhang Xiao-fen
Abstract
Zhang Xiao-fen
Abstract
Three kinds of extracts as homogenate,protein and polysaccharide were extracted from Carambola fruit.The scavenging activities of these extracts on oxygen free radical were determined by chemiluminometry.The results showed that the scavenging activities of the homogenate on hydroxy radical and hydrogen dioxide were similar,while the scavenging activity was low on superoxide anion radical.But referring to superoxide anion radical,its IC50 was about four times of above the others.The scavenging activities of the protein on superoxide anion radical and hydroxy radical were similar,while the scavenging activity was lower on hydrogen dioxide obviously.But referring to hydrogen dioxide,its IC50 was about nine times of above the others.The scavenging activity of the polysaccharide on hydroxy radical was the highest in the three kinds of oxygen free radicals.Its IC50 was about one twenty seconds of that on superoxide anion radical,and about one sixty-fifths of that on hydrogen dioxide.Results also showed that Carambola extracts could scavenge superoxide anion radical,hydroxyl radical and hydrogen peroxide.And the scavenging activities of the different Carambola extracts varied on the different oxygen free radicals.
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Three kinds of extracts as homogenate,protein and polysaccharide were extracted from Carambola fruit.The scavenging activities of these extracts on oxygen free radical were determined by chemiluminometry.The results showed that the scavenging activities of the homogenate on hydroxy radical and hydrogen dioxide were similar,while the scavenging activity was low on superoxide anion radical.But referring to superoxide anion radical,its IC50 was about four times of above the others.The scavenging activities of the protein on superoxide anion radical and hydroxy radical were similar,while the scavenging activity was lower on hydrogen dioxide obviously.But referring to hydrogen dioxide,its IC50 was about nine times of above the others.The scavenging activity of the polysaccharide on hydroxy radical was the highest in the three kinds of oxygen free radicals.Its IC50 was about one twenty seconds of that on superoxide anion radical,and about one sixty-fifths of that on hydrogen dioxide.Results also showed that Carambola extracts could scavenge superoxide anion radical,hydroxyl radical and hydrogen peroxide.And the scavenging activities of the different Carambola extracts varied on the different oxygen free radicals.
Key concepts: Superoxide, Chemistry, Radical, Hydroxyl radical, Hydrogen peroxide, Scavenging, Reactive oxygen species, Oxygen