Application of Functional Degumming Bacteria in Hemp Retting

Functional hemp degumming strains HDDMM02 and HDDMG05 isolated in laboratory were cultured to maximum enzyme production phase and inoculated for degumming and retting of Cannabis sativa fiber.The chemical composition of hemp fiber was determined;and it was showed that the optimum inoculation dose of the 2 strains was 0.5% of the volume of raw hemp;the optimal dose of urea and NaCl was 1.0 g/L.Pilot test was conducted under these conditions and indexes of hemp fiber was measured.The result showed that adding retting bacteria could increase hemp fiber yield by 12.44%,enhance hemp fiber strength by 23.67 N,improve fiber breakage strength by 0.21 cN/dtex,and increase hemp-fiber-bundle effective length by 0.16 cm.Compared to CK,SEM images showed the gum could be completely removed from the hemp with the bacteria adhered to the fiber surface;and single fiber was nearly dissociated in treatment adding degumming fiber.