Damage effect of UVB on HaCaT cells

Objective To investigate the effect of UVB in different dosage on injury of HaCaT cells and find a suitable way to construct model of injured by UVB for empirical study.Methods Cultured HaCaT cells were exposed to 10,30,50,70,90 mJ/cm2 UVB and subsequently incubated for 24 hours.Light microscope to observe the structure of HaCaT cell.MTT assay was used to test the viability of HaCat cell.A specific ELISA to detect the concentration of tumor necrosis factor-α,and a flow cytometry was used to measured apoptotic rate.Results The cellular structure underwent severe damage due to UVB irradiation.UVB irradiation reduced the viability of HaCat cell(P0.01),elevated the level of the concentration of tumor necrosis factor-α(P0.01),enhanced the apoptotic rate of HaCaT cells(P0.01).Conclusion The injury of UVB treated HaCaT cells appeared in dose-dependent,we can construct different models as we need from it.On the other way,UVB is able to induced the viability and apoptosis of HaCaT cells,elevation the level of tumor necrosis factor-α.