2011•Chinese Journal of Aesthetic MedicineRequires access

Effect of HaCaT cells proliferation by Ultraviolet-B Radiation

Jian Yang

Abstract

Objective To investigate the effect of damadge on HaCaT cells irradiated by Ultraviolet-B Radiation(UVB)(0、30、60、90mJ/cm2).Methods The dose-and time-dependent photodamage was observed by light microscopy.MTT assay was used to record cellular activity.Results The irradiation damage of HaCaT cells was dependent on the irradiated dosages(0、 30、60、90mJ/cm2).Conclusion UVB can induce damadge of HaCaT cells.

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Key concepts: HaCaT, Irradiation, Ultraviolet, MTT assay, Ultraviolet irradiation, Chemistry, Molecular biology, Cell growth