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Cloning and sequencing of interleukin-17 gene from Huiyang chicken

Mingquan Xie

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Abstract

The cDNA sequence encoding chicken interleukin-17 (ChIL-17) was cloned from the spleenic lymphocytes of Huiyang chicken by means of using the specific primers based on the reported sequence in GenBank. The full length of the cloned cDNA was 725 bp with an ORF ranging from 2nd to 508th bp, which encodes 169 amino acids. The sequence of the ChIL-17 gene had 3 bases variation aligned with the GenBank sequence and with an identity of 99.69%(722/725). The genomic DNA of this ChIL-17 gene was cloned using the same pair of primers, and is 1934 bp in its full length with 2 introns and 3 exons.

About this research paper

What this paper is about

The cDNA sequence encoding chicken interleukin-17 (ChIL-17) was cloned from the spleenic lymphocytes of Huiyang chicken by means of using the specific primers based on the reported sequence in GenBank. The full length of the cloned cDNA was 725 bp with an ORF ranging from 2nd to 508th bp, which encodes 169 amino acids. The sequence of the ChIL-17 gene had 3 bases variation aligned with the GenBank sequence and with an identity of 99.69%(722/725). The genomic DNA of this ChIL-17 gene was cloned using the same pair of primers, and is 1934 bp in its full length with 2 introns and 3 exons.

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Available abstract

The cDNA sequence encoding chicken interleukin-17 (ChIL-17) was cloned from the spleenic lymphocytes of Huiyang chicken by means of using the specific primers based on the reported sequence in GenBank. The full length of the cloned cDNA was 725 bp with an ORF ranging from 2nd to 508th bp, which encodes 169 amino acids. The sequence of the ChIL-17 gene had 3 bases variation aligned with the GenBank sequence and with an identity of 99.69%(722/725). The genomic DNA of this ChIL-17 gene was cloned using the same pair of primers, and is 1934 bp in its full length with 2 introns and 3 exons.

Key concepts: GenBank, Biology, Complementary DNA, Gene, Exon, Genetics, Cloning (programming), Intron

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