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2005•Progress in Veterinary MedicineRequires access

Molecular Cloning of cDNA Encoding Chicken Interleukine-15

YE Xiu-hua

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Abstract

Interleukin-15(IL-15) is a recently identified growth and differentiation factor with an important potential role in the initial immune response. To enable the study of the role of this cytokine in the protective immune-mechanisms generated against coccidiosis and other infectious diseases of chicken, cDNA was cloned using a pair of specific primers designed after the chicken IL-15(ChIL-15) sequence in GenBank from the actived spleenic lymphocytes isolated from the chicken treated with ConA. The full sequence of this ChIL-15 cDNA is 655 bp. Its ORF is 561 bp in length ranged from its 84th base to 644th base and encoding a protein with 187 amino acids. The sequence of this ChIL-15 cDNA is fully identical with the reported ChIL-15 as aligned by DNAsistV1.02.

About this research paper

What this paper is about

Interleukin-15(IL-15) is a recently identified growth and differentiation factor with an important potential role in the initial immune response. To enable the study of the role of this cytokine in the protective immune-mechanisms generated against coccidiosis and other infectious diseases of chicken, cDNA was cloned using a pair of specific primers designed after the chicken IL-15(ChIL-15) sequence in GenBank from the actived spleenic lymphocytes isolated from the chicken treated with ConA. The full sequence of this ChIL-15 cDNA is 655 bp. Its ORF is 561 bp in length ranged from its 84th base to 644th base and encoding a protein with 187 amino acids. The sequence of this ChIL-15 cDNA is fully identical with the reported ChIL-15 as aligned by DNAsistV1.02.

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Available abstract

Interleukin-15(IL-15) is a recently identified growth and differentiation factor with an important potential role in the initial immune response. To enable the study of the role of this cytokine in the protective immune-mechanisms generated against coccidiosis and other infectious diseases of chicken, cDNA was cloned using a pair of specific primers designed after the chicken IL-15(ChIL-15) sequence in GenBank from the actived spleenic lymphocytes isolated from the chicken treated with ConA. The full sequence of this ChIL-15 cDNA is 655 bp. Its ORF is 561 bp in length ranged from its 84th base to 644th base and encoding a protein with 187 amino acids. The sequence of this ChIL-15 cDNA is fully identical with the reported ChIL-15 as aligned by DNAsistV1.02.

Key concepts: Complementary DNA, GenBank, Biology, Cloning (programming), Molecular biology, Immune system, Genetics, Gene

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