Establishment of regeneration system of Lilium 'Siberia'

The bulb scales of the oriental lily 'Siberia' were treated as initial explants to test the inducement of bud,the bulb formation,the regeneration of leaf and leafstalk and rooting under different hormone concertrations.The result indicated that the optimal medium for adventitious bud came to be MS+6-BA 2.0 mg/L+NAA 0.5 mg/L with the differentiation rate of 85%.The most appropriate culture medium for multiplication was MS+6-BA 0.7 mg/L+NAA 0.2 mg/L,and the multiplication coefficient of bud was 3.83.The best transformation medium from adventitious bud to bulb was MS+6-BA 0.2 mg/L+NAA 0.5 mg/L+7% sugar,and the multiplication coefficient was 2.47.The most appropriate regeneration medium of scale,leaf was MS+6-BA 0.5 mg/L+2.4-D 1.0 mg/L,with the differentiation rate of 92.5%.And the culture medium MS+6-BA 1.0 mg/L+NAA 0.5 mg/L was the regeneration of leafstalk the most proper with the differentiation rate of 83.3%.Dark training showed no obvious difference on the regeneration of bulb scale,leaf and leafstalk,for the differentiation rates all came to be over 80%.And finally,the most appropriate culture medium for rooting was 1/2MS+NAA 0.2 mg/L,all of them were rooted under the treatment.