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2011•Zhonghua zhongyiyao xuekanRequires access

Study on Quality Standards of Qingyan Lotion

Liu XiaoYu

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Abstract

Objective: To establish the quality standards of Qingyan lotion.Methods:To use osthole as reference and toluene-ethylacetate(30:1) as developer for identify Fructus Cnidii by TLC.To use matrine as reference and toluene-ethylacetate-acetone-ammonia test solution(2:4:3:0.2)as developer for identify Radix Sophorae Flavescentis byTLC.RP-HPLC was used to determine osthole in Fructus cnidii.Results: The TLC spots were clear.The reference substance of osthole had a good linear relationship in the range of 4.75~23.75μg/mL,the regression equation was C=0.0131A-0.322,r=0.9997(n=5),the average recovery rate was 95.26%,RSD=1.55%(n=9).Conclusion: The method is simple and accurate with good reproducibility and can be applied to the quality control of Qingyan lotion.

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What this paper is about

Objective: To establish the quality standards of Qingyan lotion.Methods:To use osthole as reference and toluene-ethylacetate(30:1) as developer for identify Fructus Cnidii by TLC.To use matrine as reference and toluene-ethylacetate-acetone-ammonia test solution(2:4:3:0.2)as developer for identify Radix Sophorae Flavescentis byTLC.RP-HPLC was used to determine osthole in Fructus cnidii.Results: The TLC spots were clear.The reference substance of osthole had a good linear relationship in the range of 4.75~23.75μg/mL,the regression equation was C=0.0131A-0.322,r=0.9997(n=5),the average recovery rate was 95.26%,RSD=1.55%(n=9).Conclusion: The method is simple and accurate with good reproducibility and can be applied to the quality control of Qingyan lotion.

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Available abstract

Objective: To establish the quality standards of Qingyan lotion.Methods:To use osthole as reference and toluene-ethylacetate(30:1) as developer for identify Fructus Cnidii by TLC.To use matrine as reference and toluene-ethylacetate-acetone-ammonia test solution(2:4:3:0.2)as developer for identify Radix Sophorae Flavescentis byTLC.RP-HPLC was used to determine osthole in Fructus cnidii.Results: The TLC spots were clear.The reference substance of osthole had a good linear relationship in the range of 4.75~23.75μg/mL,the regression equation was C=0.0131A-0.322,r=0.9997(n=5),the average recovery rate was 95.26%,RSD=1.55%(n=9).Conclusion: The method is simple and accurate with good reproducibility and can be applied to the quality control of Qingyan lotion.

Key concepts: Lotion, Chromatography, Recovery rate, Quality standard, Acetone, Linear relationship, Matrine, Toluene

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