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2012•Journal of Inner Mongolia UniversityRequires access

Interaction of Benzbromarone with Bovine Serum Albumin:Investigation by Fuorescence Spectroscopy

Xiaoxia Wang

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Abstract

Effects of benzbromarone(BXML) and Cu(Ⅱ) exist respectively or simultaneously on the fluorescence spectrum characteristics of bovine serum albumin(BSA)were studied.The results showed that both Cu(Ⅱ) and BXML could quench the fluorescence intensity of BSA via a nonradiative energy transfer mechanism.When Cu(Ⅱ) was present,BXML could quench the fluorescence of BSA more significantly.The binding constants(K) and the binding sites(n) were calculated by analyzing fluorescence quenching data with double-reciprocal equation.The K and n between BXML and BSA were 9.54×105L/mol and 1.03,those between Cu(Ⅱ) and BSA were 1.13×103 L/mol and 0.74 respectively.

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What this paper is about

Effects of benzbromarone(BXML) and Cu(Ⅱ) exist respectively or simultaneously on the fluorescence spectrum characteristics of bovine serum albumin(BSA)were studied.The results showed that both Cu(Ⅱ) and BXML could quench the fluorescence intensity of BSA via a nonradiative energy transfer mechanism.When Cu(Ⅱ) was present,BXML could quench the fluorescence of BSA more significantly.The binding constants(K) and the binding sites(n) were calculated by analyzing fluorescence quenching data with double-reciprocal equation.The K and n between BXML and BSA were 9.54×105L/mol and 1.03,those between Cu(Ⅱ) and BSA were 1.13×103 L/mol and 0.74 respectively.

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Available abstract

Effects of benzbromarone(BXML) and Cu(Ⅱ) exist respectively or simultaneously on the fluorescence spectrum characteristics of bovine serum albumin(BSA)were studied.The results showed that both Cu(Ⅱ) and BXML could quench the fluorescence intensity of BSA via a nonradiative energy transfer mechanism.When Cu(Ⅱ) was present,BXML could quench the fluorescence of BSA more significantly.The binding constants(K) and the binding sites(n) were calculated by analyzing fluorescence quenching data with double-reciprocal equation.The K and n between BXML and BSA were 9.54×105L/mol and 1.03,those between Cu(Ⅱ) and BSA were 1.13×103 L/mol and 0.74 respectively.

Key concepts: Bovine serum albumin, Chemistry, Fluorescence, Quenching (fluorescence), Analytical Chemistry (journal), Fluorescence spectroscopy, Benzbromarone, Spectroscopy

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