ScholarLens Back to search
2003•Unpublished venueRequires access

Study of Binding Competition between Cu(II) and Zn(II) to Bovine Serum Albumin by Fluorescence Quenching

Hong Liang

Open publisher page 5 citations

Abstract

Fluorescence quenching of bovine serum albumin (BSA) by its interaction with Cu(Ⅱ) and Zn(Ⅱ) or with Cu(Ⅱ) alone was observed at 28℃ and physiologically pH 7.43(±0.02) applying fluorescence spectra method. The Stern-Volmer's K'sv cu.BSA, and Lineweaver-Burk's K'D CU-BSA were calculated in these two kinds of systems. The different interactions of Cu(Ⅱ) and Zn(Ⅱ) with BSA were discussed in this paper. By contrasting the single metal system with the double-metal system, the binding of Cu(Ⅱ) and BSA must be strengthened by the competition of Zn(Ⅱ).

About this research paper

What this paper is about

Fluorescence quenching of bovine serum albumin (BSA) by its interaction with Cu(Ⅱ) and Zn(Ⅱ) or with Cu(Ⅱ) alone was observed at 28℃ and physiologically pH 7.43(±0.02) applying fluorescence spectra method. The Stern-Volmer's K'sv cu.BSA, and Lineweaver-Burk's K'D CU-BSA were calculated in these two kinds of systems. The different interactions of Cu(Ⅱ) and Zn(Ⅱ) with BSA were discussed in this paper. By contrasting the single metal system with the double-metal system, the binding of Cu(Ⅱ) and BSA must be strengthened by the competition of Zn(Ⅱ).

Why it matters

OpenAlex reports 5 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Fluorescence quenching of bovine serum albumin (BSA) by its interaction with Cu(Ⅱ) and Zn(Ⅱ) or with Cu(Ⅱ) alone was observed at 28℃ and physiologically pH 7.43(±0.02) applying fluorescence spectra method. The Stern-Volmer's K'sv cu.BSA, and Lineweaver-Burk's K'D CU-BSA were calculated in these two kinds of systems. The different interactions of Cu(Ⅱ) and Zn(Ⅱ) with BSA were discussed in this paper. By contrasting the single metal system with the double-metal system, the binding of Cu(Ⅱ) and BSA must be strengthened by the competition of Zn(Ⅱ).

Key concepts: Bovine serum albumin, Chemistry, Quenching (fluorescence), Fluorescence, Metal, Zinc, Copper, Analytical Chemistry (journal)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study of Binding Competition between Cu(II) and Zn(II) to Bovine Serum Albumin by Fluorescence Quenching — Research Paper | ScholarLens