Detection of Id1 mRNA expression in prostate cancer by real time RT-PCR

Objective To investigate the mRNA expression of Id1(inhibitor of DNA binding or inhibitor of differentiation)in human prostate cancer and its clinical significance.Methods The quantity of Id1 mRNA relative to b-actin was detected by SYBR GreenⅠ-based real time RT-PCR in prostatectomy or biopsy samples of human normal prostate,benign prostate hyperplasia(BPH)and prostate cancer sam- ples.The relationship between the quantity of Id1 mRNA and some clinieopathological parameters,such as Gleason grade,prostate specific antigen(PSA)and clinical stage,was further analyzed.Results In pros- tate cancer tissues,the mean quantity of Id1 mRNA relative to b-actin was obviously higher than that in BPH(P0.01).The expression of Id1 mRNA was positively correlated with Gleason grade in prostate cancer group(r=0.9995,P0.05).One to two years after therapeutic prostateetomy,the probability of infiltration and relapse was obviously higher in the patients whose Id1 mRNA expression were markedly up- regulated.Conclusion The expression of Id1 mRNA is significantly increased in prostate eaneer,and it is negatively correlated with the differentiation level of prostate cancer.The high expression of Id1 mRNA re- fleeted its relationship with the progression of prostate cancer on the level of transcription.