Expression of heat shock protein 70 in rat spinal cord with acute contusion

Objective To observe the expression of heat shock protein 70(HSP 70)in rat spinal cord with acute contusion.Methods Sixty-five SD rats were divided into 3 groups:5 in control group,30 in operative group,30 in spinal cord injury group.The injuries of spinal cord were induced by Allen's model.Spinal cords observed in operative group were collected from 5 rats each time at 6 time points(2 h,6 h,12 h,24 h,48 h and 72 h after management),as same as the spinal cord injury group.HSP 70 expression in spinal cords from each group at different time points was studied by method of immunohistochemistry.Results There was not constitutive expression of HSP 70 in the normal spinal cord.But expression of HSP 70 had been already found at 2 h after the spinal cord contusion,reached the peak at 24～48 h,and could been observed at 72 h after contusion.Conclusion Expression of HSP 70 is a part of stress reaction for organism.HSP 70 obviously increased in gray nucleus of spinal cord from 2 h to 72 h after acute injury.HSP 70 maybe make for preventing secondary lesion of spinal cord.